Larry Helseth , PhD

• Undergraduate: BS, Biochemistry, Texas Christian University, Ft. Worth, TX
• Graduate: PhD, Biochemistry, Northwestern University, Evanston, IL
• Postgraduate: Department of Biochemistry, UMDNJ-Rutgers Medical School, Piscataway, NJ
  Department of Oral Biology, Northwestern University Dental School, Chicago, IL
  School of Computer Science, DePaul University, Chicago, IL

• Genomics
• Proteomics
• Systems Biology
• Applying a multi-omics approach to disease

After post-doctoral training he taught at Northwestern University Dental School for 5 years before moving to industry where he worked for 14 years for Baxter Healthcare Corporation.  After leaving Baxter he received post-doctoral training in bioinformatics at De Paul University.   He then helped run the Proteomics and Informatics Services Facility at the University of Illinois at Chicago before joining NorthShore as a Bioinformatician in 2012. Helseth was a part of the team that developed NorthShore’s software for Personalized Medicine, Flype, which is used for analyzing DNA sequence data for Personalized Medicine and Pharmacogenomics.

• National Merit Scholar
• NIH Postdoctoral Fellowship: Individual National Research Service Award
• Member, Special Grants Review Committee, NIDR

• Special Grants Review Committee, NIDR, 1987-1991
• Co-course Director and Instructor, CBC Proteomics and Informatics Workshop, University of Illinois at Chicago, 2007-2013
• Chicago Mass Spec Discussion Group, Board member, 2011-present

Publications in Peer-Review Journals:

1. Flype: Software for enabling personalized medicine.
   Helseth DL Jr, Gulukota K, Miller N, Yang M, Werth T, Sabatini LM, Bouma M, Dunnenberger HM, Wake DT, Hulick PJ, Kaul KL, Khandekar JD.
   Am J Med Genet C Semin Med Genet. 2021 Mar;187(1):37-47. doi: 10.1002/ajmg.c.31867. Epub 2020 Dec 3. PMID: 33270363
2. LyP-1-Modified Oncolytic Adenoviruses Targeting Transforming Growth Factor β Inhibit Tumor Growth and Metastases and Augment Immune Checkpoint Inhibitor Therapy in Breast Cancer Mouse Models.
   Xu W, Yang Y, Hu Z, Head M, Mangold KA, Sullivan M, Wang E, Saha P, Gulukota K, Helseth DL, Guise T, Prabhkar BS, Kaul K, Schreiber H, Seth P.
   Hum Gene Ther. 2020 Aug;31(15-16):863-880. doi: 10.1089/hum.2020.078. Epub 2020 Jun 12. PMID: 32394753
3. An Oncolytic Adenovirus Targeting Transforming Growth Factor β Inhibits Protumorigenic Signals and Produces Immune Activation: A Novel Approach to Enhance Anti-PD-1 and Anti-CTLA-4 Therapy.
   Yang Y, Xu W, Peng D, Wang H, Zhang X, Wang H, Xiao F, Zhu Y, Ji Y, Gulukota K, Helseth DL Jr, Mangold KA, Sullivan M, Kaul K, Wang E, Prabhakar BS, Li J, Wu X, Wang L, Seth P.
   Hum Gene Ther. 2019 Sep;30(9):1117-1132. doi: 10.1089/hum.2019.059. Epub 2019 Jul 1. PMID: 31126191
4. Na R, Zheng SL, Han M, Yu H, Jiang D, Shah S, Ewing CM, Zhang L, Novakovic K, Petkewicz J, Gulukota K, Helseth DL Jr, Quinn M, Humphries E, Wiley KE, Isaacs SD, Wu Y, Liu X, Zhang N, Wang CH, Khandekar J, Hulick PJ, Shevrin DH, Cooney KA, Shen Z, Partin AW, Carter HB, Carducci MA, Eisenberger MA, Denmeade SR, McGuire M, Walsh PC, Helfand BT, Brendler CB, Ding Q, Xu J, Isaacs WB. “Germline Mutations in ATM and BRCA1/2 Distinguish Risk for Lethal and Indolent Prostate Cancer and are Associated with Early Age at Death.” Eur Urol. 2016 Dec 9. pii: S0302-2838(16)30885-5. doi: 10.1016/j.eururo.2016.11.033. [Epub ahead of print]  PMID: 27989354
5. Zhu Y*, Xu Y*, Helseth DL Jr*, Gulukota K, Yang S, Pesce LL, Mitra R, Müller P, Sengupta S, Guo W, Silverstein JC, Foster I, Parsad N, White KP, Ji Y. “Zodiac: A Comprehensive Depiction of Genetic Interactions in Cancer by Integrating TCGA Data.” J Natl Cancer Inst. 2015 May 8;107(8). pii: djv129. doi: 10.1093/jnci/djv129. Print 2015 Aug. PMID: 25956356 (*Co-first authors).
6. Gulukota K, Helseth DL Jr, Khandekar JD.   “Direct observation of genomic heterogeneity through local haplotyping analysis.”  BMC Genomics. 2014 Jun 2;15(1):418. [Epub ahead of print]  PMID: 24888354.
7. Song Y, Kirkpatrick LL, Schilling AB, Helseth DL, Chabot N, Keillor JW, Johnson GV, Brady ST, “Transglutaminase and Polyamination of Neuronal Tubulins ---A Novel Posttranslational Modification for Stabilizing Microtubules”, Neuron. 2013 Apr 10;78(1):109-23.  PMID: 23583110.
8. Sabherwal Y, Mahajan N, Helseth DL, Gassmann M, Shi H, Zhang M. “PDEF downregulates stathmin expression in prostate cancer.” Int J Oncol. 2012 Feb 29. doi: 10.3892/ijo.2012.1392. [Epub ahead of print] PMID: 22378487.
9. Avner, B.S., Shioura, K.M., Scruggs, S.B., Geenen, D.L., Helseth, D.L., Farjah, M., Goldspink, P and Solaro, R.J.  “Myocardial Infarction in Mice Alters Sarcomeric Function Via Post-Translational Protein Modification.” Mol Cell Biochem. 2012 Apr;363(1-2):203-15. Epub 2011 Dec 8. PMID: 22160857.
10. Aguirre B, Costas M, Cabrera N, Mendoza-Hernández G, Helseth DL Jr., Fernández P, de Gómez-Puyou MT, Pérez-Montfort R, Torres-Larios A, Gómez Puyou A, . 2011 “A Ribosomal Misincorporation of Lys for Arg in Human Triosephosphate Isomerase Expressed in Escherichia coli Gives Rise to Two Protein Populations.” PLoS ONE 6(6): e21035. doi:10.1371/journal.pone.0021035.
11. Markaryan, A, Nelson, EG, Helseth, DL, and Hinojosa, R, “Proteomic Analysis of Formalin Fixed Celloidin Embedded Whole Cochlear and Laser Microdissected Spiral Ganglion Tissues”, Acta Oto-Laryngologica, 130(2010):984-9 PMID: 20148753.
12. Warren, CM, Geenen, DL, Helseth, DL Jr., Xu, H, and Solaro, RJ, “Sub-Proteomic fractionation, iTRAQ, and OFFGEL-LC-MS/MS approaches to cardiac proteomics”, Journal of Proteomics, 73(2010):1551-61. PMID: 20394843
13. Parker L, Engel-Hall A, Drew K, Steinhardt G, Helseth DL Jr, Jabon D, McMurry T, Angulo DS, Kron SJ. "Investigating quantitation of phosphorylation using MALDI-TOF mass spectrometry" J Mass Spectrom. 43 (2008):518-27. PMID: 18064576.
14. Helseth DL Jr, Tolwin TM, Kaminski EJ, Osetek EM “Incomplete polymerization of Cavalite with the use of recommended photopolymerization times: a warning of possible cytotoxic effects.”  Oral Surg Oral Med Oral Pathol. 1989 Aug;68(2):223-5.
15. Helseth DL Jr, Veis A.  “Cathepsin D-mediated processing of procollagen: lysosomal enzyme involvement in secretory processing of procollagen.”  Proc Natl Acad Sci U S A. 1984 Jun;81(11):3302-6.
16. Helseth, D. L. Jr., and Veis, A., "Conformational studies on the telopeptides of collagen.  Implications for intermolecular interactions", in The Chemistry and Biology of Mineralized Connective Tissues, A. Veis, ed., Elsevier North Holland, Inc., 1981, pp. 85-89.
17. Helseth DL Jr, Veis A.  “Collagen self-assembly in vitro. Differentiating specific telopeptide-dependent interactions using selective enzyme modification and the addition of free amino telopeptide.”  J Biol Chem. 1981 Jul 25;256(14):7118-28.
18. Helseth, D. L. Jr., Lechner, J. H., and Veis, A., "The role of the amino-terminal extrahelical region of Type I collagen in directing the 4 D overlap in fibrillogenesis", Biopolymers, 18 (1979): 3005-3014.
19. Babitch, J. A., Helseth, D. L., and Chiu, T.-C., "On using cis-Pt (II)-uracil as a stain for nucleic acids in brain slices and subcellular fractions", Histochem., 49 (1976): 253-261.
20. Babitch, J. A., Breithaupt, T. B., Chiu, T.-C., Garadi, R., and Helseth, D. L. Jr., "Preparation of chick brain synaptosomes and synaptosomal membranes", Biochim. Biophys. Acta, 433 (1976): 75-89.

Book Chapters:

1. Helseth, D. L. Jr., and Veis, A., "Conformational studies on the telopeptides of collagen.  Implications for intermolecular interactions", in The Chemistry and Biology of Mineralized Connective Tissues, A. Veis, ed., Elsevier North Holland, Inc., 1981, pp. 85-89.

Abstracts:

1. Schilling, A.B., Crot, C., Helseth, L., Davis, R., Xu, H., “Conversion of an Agilent Chip Cube system and adaptation of a ROXY EC potentiostat for the analysis of proteolytic and non-proteolytic protein samples on a Thermo Finnigan LTQ-FT Ultra mass spectrometer.” Poster presentation, Assoc. Biomolecular Research Facilities (ABRF) 2010 Meeting, Sacramento, CA, March 20-23, 2010.
2. Avner, B. S., Shioura, K. M., Scruggs, S. B., Helseth, D. L., Goldspink, P. H., and Solaro, R.J., “Alterations to cardiac muscle function and sarcomeric proteins following myocardial infarction in mice”, Biophysics Society 2010 Meeting, Feb. 20 - 24, 2010, San Francisco, CA
3. Warren, C.M., Geenen, D.L., Helseth, D. L. Jr., R. John Solaro, R. J., “Sub-proteomic Fractionation of Rat Cardiac Tissue: Comparing Ischemic vs Normal Remote Region With In-solution Based Proteomics”, Biophysics Society 2010 Meeting, Feb. 20 - 24, 2010, San Francisco, CA.
4. Helseth, D. L. Jr., Xu, H., Schilling, A. B.  “Automating Commercial Mass Spectrometry Search Engines.” Poster presentation, Assoc. Biomolecular Research Facilities (ABRF) 2009 Meeting, Memphis, TN, February 7-10, 2009.
5. Xu, H., Helseth, D. L. Jr., Schilling, A. B., Freitas, M. A. “A Free Web-Based Database Search Engine for Tandem Mass Spectrometric Data of Proteins and Peptides.” Poster presentation ABRF 2009 Meeting, Memphis, TN, February 7-10, 2009
6. Kristjiansdottir, K, Wolfgeher, D., Helseth D.L. Jr., Lucius, N, Angulo, D.S.,  and Kron, S.J. "Phosphoprotein profiling: Cell cycle regulated phosphoproteins and complexes in yeast. American Association for Cancer Research Special Conference: Advances in Proteomics in Cancer Research, Amelia Island, Florida, February 27 - March 2, 2007
7. Helseth, D. L. Jr., "Isolation of collagenase from human periodontal ligament fibroblasts", FASEB J., 2 (March 1988): A1754.
8. Dooley, S. L., and Helseth, D. L. Jr., "In vitro swelling properties of Wharton's jelly", Society for Gynecologic Investigation, March 1986, Toronto (poster presentation).
9. Dooley, S. L., Lamb, R., and Helseth, D. L. Jr., "Umbilical cord circumference as a measure of Wharton's jelly: Clinical correlates" Society of Perinatal Obstetricians, January 1986, Dallas (poster presentation).
10. Helseth, D. L. Jr., "Cleavage of procollagen by Cathepsin D- Evidence for a physiological role.", J. Cellular Biochem., 8B (1984)
11. Veis A., and Helseth, D. L., "Polyelectrolyte-collagen interactions", Connective Tissue Research, 11 (1983): 239.
12. Helseth, D. L. Jr., and Veis, A., "Evidence for a lysosomal (Catheptic) pathway in procollagen COOH-propeptide removal."  Fed. Proc., 42 (1983): 1888.
13. Helseth, D. L. Jr., and Veis, A., "Collagen self-assembly:  Role of telopeptides in in vitro fibril formation", Fed. Proc., 38 (1979): 819.

Publications