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Wang, San Ming, MD

Director
Center for Functional Genomics

Undergraduate:  Bachelors of Medicine, Jiangxi Medical College, Jiangxi, China
Masters of Medicine-Immunology, Shandong Medical University, Shandong, China
Medical School:  M.D., Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland
Post-Doctoral Fellowship : Gene Expression, Northwestern University and University of Chicago  

Research Interests:  
Dr. Wang's laboratory is developing and applying multiple genome technologies for human genome and cancer genome analysis, with the aims to identify novel genes in the human genome, to identify cancer-related genes and to determine the structural abnormalities in the cancer genome.

Major Achievements Over the Past Years:

  • Developed an efficient approach for novel gene identification--SM Wang, SC Fears, L Zhang, J-J Chen, JD Rowley. Screening poly dA/dT(-) cDNAs for gene identification. Proc. Natl. Acad. Sci. USA 97: 4162-4167, 2000. COMMENTARY by KJ Martin and AB Pardee. Identifying expressed genes. Proc. Natl. Acad. Sci. USA  97: 3789-3791, 2000
  • Develop an approach for novel transcript identification using SAGE tags--J Chen, S Lee, G Zhou, SM Wang. High-throughput GLGI procedure for converting a large number of serial analysis of gene expression tag sequences into 3' complementary DNAs. Genes, Chromosomes & Cancer 33:252-261, 2002
  • Identified a flaw in misinterpreting SAGE data in transcriptome study--S Lee, T Clark, J Chen, G Zhou, LR Scott, JD Rowley, SM Wang. Correct identification of genes from SAGE tag sequences. Genomics 79:598-599, 2002
  • Identified a systematic flaw in transcriptome study that internal priming generates many truncated sequences-- D Nam, S Lee, G Zhou, X Cao, C Wang, J Chen, T Clark, JD Rowley, SM Wang. Oligo dT primer generates a high frequency of truncated cDNAs through internal poly A priming during reverse transcription. Proc. Natl. Acad. Sci. USA. 99:6152-6156, 2002
  • Determined the gene expression pattern in B cell commitment--M Müschen, S Lee, G Zhou, N Feldhahn, VS Barath, J Chen, C Moers, M Krönke, JD Rowley, SM Wang. Molecular portraits of B cell lineage commitment. Proc. Natl. Acad. Sci. USA 99:10014-10019, 2002
  • Determined that there are far more novel transcripts and genes in the human genome-- J Chen, M Sun, S Lee, G Zhou, JD Rowley, SM Wang. Identifying novel transcripts and novel genes in the human genome by using novel SAGE tags. Proc. Natl. Acad. Sci. USA 99:12257-12262, 2002
  • Theoretically predicted that SAGE tags can be used for novel gene identification--SM Wang. The new role of SAGE in gene discovery. Trends in Biotechnology 21:57-58, 2003
  • Determined that the high sensitivity of SAGE is the reason for detecting more novel transcripts--M Sun, G Zhou, S Lee, J Chen, RZ Shi, JD Rowley, SM Wang. SAGE is far more sensitive than EST for detecting low abundant transcripts. BMC Genomics 5:1-4, 2004
  • Provided a genome-level experimental evidence showing that the existence of novel transcripts in the Drosophila genome--S Lee, J Bao, G Zhou, J Shapino, J Xu, RZ Shi, X Lu, T Clark, D Johnson, YC Kim, C Wing, C Tseng, M Sun, W Lin, J Wang, W Du, C-I Wu, X Zhang, SM Wang. Detecting novel low-abundant transcripts in Drosophila. RNA 11:939-946, 2005
  • Provided first set of genome-level evidence that the radiafrequency can cost cells to alter its gene expression patterns--S Lee, D Johnson, K Dunbar, H Dong, X Ge, YC Kim, C Wing, N Jayathilaka, N Emmanue, CQ Zhou, HL Gerber, CC Tseng, SM Wang. 2.45 GHz Radiofrequency fields alter gene expression in cultured human cells. FEBS Letters, 579:4829-4836, 2005
  • Edited a book that provides a comprehensive summary for SAGE studies-SAGE: Current Technologies and Applications. p1-375. Horizon Scientific Press, UK. Editor: SM Wang, 2005
  • Developed a system for determining the gene origin for ambiguous SAGE tags--X Ge, YC Jung, Q Wu, WA Kibbe, SM Wang. Annotating non-specific SAGE tags with microarray data, Genomics, 87:173-180, 2006
  • Determined the gene expression patterns in AML cells--S Lee, J Chen, G Zhou, RZ Shi, G Bouffard, M Kocherginsky, X Ge, M Sun, N Jayathilaka, YC Kim,  N Emmanuel, S Bohlander, M Minden, J Kline, O Ozer, R Larson, M LeBeau, E Green,  J Trent, T Karrison, P Liu, SM Wang, JD Rowley. Gene Expression Profiles in Acute Myeloid Leukemia with Common Translocations using SAGE. Proc. Natl. Acad. Sci. USA 103:1030-1035, 2006
  • Identified a third more antisense transcripts in the human genome--Ge X, Wu Q, Jung YC, Chen J, SM Wang. A large quantity of novel human antisense transcripts detected by LongSAGE. Bioinformatics. 2006 PMID: 16895931

Selected Publications (>2005):

  1. S Lee, J Bao, G Zhou, J Shapino, J Xu, RZ Shi, X Lu, T Clark, D Johnson, YC Kim, C Wing, C Tseng, M Sun, W Lin, J Wang, W Du, C-I Wu, X Zhang, SM Wang. Detecting novel low-abundant transcripts in Drosophila. RNA 11:939-946, 2005
  2. J Bao, S Lee, C Chen, XQ Zhang, Y Zhang, SQ Liu, T Clark, J Wang, ML Cao, HM Yang, SM Wang, J Yu. SAGE (Serial Analysis of Gene Expression) Study of a Hybrid Rice Strain (LYP9) and Its Parental Cultivars. Plant Physiology, 138:1216-1231, 2005
  3. X Ge, S Yamamoto, S Tsutsumi, Y Midorikawa, S Ihara, SM Wang, H Aburatani. Interpreting expression profiles of cancers by genome-wide survey of breadth of expression in normal tissues. Genomics, 86:127-141, 2005
  4. S Lee, D Johnson, K Dunbar, H Dong, X Ge, YC Kim, C Wing, N Jayathilaka, N Emmanue, CQ Zhou, HL Gerber, CC Tseng, SM Wang. 2.45 GHz Radiofrequency fields alter gene expression in cultured human cells. FEBS Letters, 579:4829-4836, 2005
  5. HS Kang, EM Kim, S Lee, SR Yoon, T Kawamura, YC Lee, S Kim, PK Myung, SM Wang, I Choi. Stage-dependent gene expression profiles during natural killer cell development. Genomics 86:551-65, 2005
  6. SM Wang. Reverse Transcriptome: Identification of Novel Transcripts Through Novel SAGE Tags. In SAGE Technologies: Current Innovations and Future Trends. Horizon Scientific Press, London. Editor: SM Wang, 365-371, 2005
  7. SAGE: Current Technologies and Applications. p1-375. Horizon Scientific Press, UK. Editor: SM Wang, 2005
  8. X Ge, YC Jung, Q Wu, WA Kibbe, SM Wang. Annotating non-specific SAGE tags with microarray data. Genomics, 87:173-180, 2006
  9. S Lee, J Chen, G Zhou, RZ Shi, G Bouffard, M Kocherginsky, X Ge, M Sun,  N Jayathilaka, YC Kim,  N Emmanuel, S Bohlander, M Minden, J Kline, O Ozer, R Larson, M LeBeau, E Green,  J Trent, T Karrison, P Liu, SM Wang, JD Rowley. Gene Expression Profiles in Acute Myeloid Leukemia with Common Translocations using SAGE. Proc. Natl. Acad. Sci. USA 103:1030-1035, 2006
  10. SM Wang. Applying the SAGE technique to study the effects of electromagnetic field on biological systems. Proteomics. 2006  PMID: 16897688
  11. Ge X, Wu Q, Jung YC, Chen J, SM Wang. A large quantity of novel human antisense transcripts detected by LongSAGE. Bioinformatics. 2006 PMID: 16895931
  12. X Ge, SM Wang. Identifying non-specific SAGE tags by context of gene expression. Methods in Molecular Biology-SAGE. Humana Press. Editor: Kåre Lehmann Nielsen, 2006 (in press)
  13. SM Wang. Understanding SAGE data. Trends in Genetics. (in press)

Contact Information:  
Telephone:  224.364.7491
Facsimilie:  224.364.5003
Email:  swang@northshore.org